| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gotham, Madison E (15)
|
NTMTRO |
56.82 |
57.20 |
0.38 |
| 2 |
Hall, Madalyn L (15)
|
NTMTRO |
1:00.69 |
57.35 |
-3.34 |
| 3 |
Dingwerth, Emmerson A (16)
|
NTMTRO |
54.60 |
58.34 |
3.74 |
| 4 |
Deitemeyer, Abby M (15)
|
NTMTRO |
59.22 |
59.66 |
0.44 |
| 5 |
Liu, Cassie B (15)
|
NTMTRO |
1:00.74 |
1:00.40 |
-0.34 |
| 6 |
Poulton, Amelia S (16)
|
NTMTRO |
1:00.45 |
1:02.62 |
2.17 |
| 7 |
McCants, Abigail F (16)
|
NTMTRO |
1:00.97 |
1:02.84 |
1.87 |
| 8 |
Welch, Claire A (17)
|
NTMTRO |
1:02.09 |
1:04.82 |
2.73 |
| 9 |
Trivedi, Shreya H (15)
|
NTMTRO |
1:03.50 |
1:05.52 |
2.02 |
| 10 |
Ohri, Misha M (15)
|
NTMTRO |
1:05.36 |
1:06.55 |
1.19 |
| 11 |
Sutherland, Maximillia E (15)
|
NTMTRO |
1:04.95 |
1:07.91 |
2.96 |
|
Erwin, Kaylee F (15)
|
NTMTRO |
1:04.89 |
NS |
- |
|
Hyland, Kerri J (15)
|
NTMTRO |
57.84 |
NS |
- |
|
Noone, Chloe L (15)
|
NTMTRO |
59.37 |
NS |
- |
|
Thompson, Talia E (16)
|
NTMTRO |
58.17 |
NS |
- |